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Cell Biology

Study Pack

Set 4 Molecular Cloning Methods

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Question 1

(Multiple Choice)

Free
A new enzyme was isolated and found to have a recognition sequence of 5 base pairs.How often will it cut in a genome?

A)64
B)4096
C)8192
D)1024
E)3124

Answer

Question 2

(Multiple Choice)

Free
One approach to prevent religation of the ends of a restricted plasmid DNA is to

A)store the plasmid in DNase.
B)store the plasmid in RNAase.
C)treat the plasmid with alkaline phosphatase to remove phosphates from the 'sticky ends'
D)treat the plasmid with a protease to remove all proteins.
E)treat the plasmid with BamHI to create gaps.

Answer

Question 3

(Multiple Choice)

Free
The complementary sequence of one of the following is a palindrome.Which one?

A)GAATTC
B)CTGACT
C)ATCCTC
D)GGCCAA
E)CCTTTC

Answer