A)It binds to Rpb3 to facilitate melting of the DNA promoter.
B)It extends the dock region of polymerase,making it easier for transcription factors to bind.
C)It binds to DNA and facilitate elongation.
D)It binds to Rpb3 to facilitate melting of the DNA promoter and it extends the dock region of polymerase,making it easier for transcription factors to bind are correct.
E)It extends the dock region of polymerase,making it easier for transcription factors to bind and it binds to DNA and facilitate elongation are correct.

A)chromatography
B)western blotting
C)S1 nuclease assay
D)DNase 1 footprinting
E)chromatography and S1 nuclease assay

A)Break the enzymes into component parts and gradually reassemble them.
B)Use mutational analysis.
C)Conduct DNA binding assays.
D)Break the enzymes into component parts and gradually reassemble them and use mutational analysis are correct.
E)Use mutational analysis and conduct DNA binding assays are correct.