Course

Cell Biology

Study Pack

Set 11 General Transcription Factors In Eukaryotes

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Question 1

(Multiple Choice)

Free
One approach to studying the phosphoryation of the CTD of RNA polymerase II would be

A)the use of photo-crosslinking.
B)the use of [ α\alpha -32]ATP in the presence of a phosphorylase.
C)the use of [ γ\gamma -32]ATP in the presence of a kinase.
D)the use of photo-crosslinking and the use of [ γ\gamma -32]ATP in the presence of a kinase.
E)the use of photo-crosslinking and the use of [ α\alpha -32]ATP in the presence of a phosphorylase.

Answer

Question 2

(Multiple Choice)

Free
Which of the following is a possible effect of creating a mutant cell line with reduced levels of TAF1?

A)Initiation complex would be slow to form.
B)Polymerase II will show weak binding to the promoter.
C)Proofreading activity will be impaired.
D)The DAB complex will dissociate.
E)None of the choices are correct.

Answer

Question 3

(Multiple Choice)

Free
Which of the following would you expect to bind to a chromatography column made of a resin conjugated to UBF?

A)Sp1
B)SL1
C)TFIIB
D)TFIIH
E)TFIIE

Answer