Molecular Tools For Studying Genes And Gene Activity - Molecular Biology | Biology+

Course

Cell Biology

Study Pack

Set 1: A Brief History

Set 2: The Molecular Nature of Genes

Set 3: An Introduction to Gene Function

Set 4: Molecular Cloning Methods

Set 5: Molecular Tools for Studying Genes and Gene Activity

Set 6: The Mechanism of Transcription in Bacteria

Set 7: Operons: Fine Control of Bacterial Transcription

Set 8: Major Shifts in Bacterial Transcription

Set 9: Dna-Protein Interactions in Bacteria

Set 10: Eukaryotic Rna Polymerases and Their Promoters

Set 11: General Transcription Factors in Eukaryotes

Set 12: Transcription Activators in Eukaryotes

Set 13: Chromatin Structure and Its Effect on Transcription

Set 14: Rna Processing I: Splicing

Set 15: Rna Processing II: Capping and Polyadenylation

Set 16: Rna Processing: Other Post-Transcriptional Events

Set 17: The Mechanism of Translation I: Initiation

Set 18: The Mechanism of Translation II: Elongation and Termination

Set 19: Ribosomes and Transfer Rna

Set 20: Dna Replication,damage and Repair

Set 21: Dna Replication II: Detailed Mechanism

Set 22: Homologous Recombination

Set 23: Transposition

Set 24: Introduction to Genomics: Dna Sequencing on a Genomic Scale

Set 25: Genomics II: Functional Genomics,proteomics and Bioinformatics

Set 5 Molecular Tools For Studying Genes And Gene Activity

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Set 5 Molecular Tools For Studying Genes And Gene Activity

(Multiple Choice)

Free

A DNA fragment was treated with EcoRI restriction enzyme.The restriction pattern is shown below.How many fragments should be visible on a Southern blot if radioactively labeled probe "1" was used?

A)0
B)1
C)2
D)3
E)4

A DNA fragment was treated with EcoRI restriction enzyme.The restriction pattern is shown below.How many fragments should be visible on a Southern blot if radioactively labeled probe 1 was used? A)0 B)1 C)2 D)3 E)4

Answer

(Multiple Choice)

Free

A technique is needed to rapidly separate positively charged histone proteins from a crude cell extract.Which of the following should be chosen?

A)SDS-PAGE
B)phosphocellulose cation-exchange chromatography
C)pulse-field gel electophoresis
D)gel filtration chromatography
E)DEAE-Sephadex chromatography

Answer

(Multiple Choice)

Free

During SDS PAGE,which of the following does not directly affect the migration of the polypeptides through the gel?

A)the size of the polypeptide
B)the strength of the electric field
C)the molecular mass of the protein
D)the native charge on the polypeptides
E)the SDS

Answer