Course

Cell Biology

Study Pack

Set 9 Dna Protein Interactions In Bacteria

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Question 1

(Multiple Choice)

Free
Which of the following techniques would you use if you were designing an experiment to look at cooperativity of repressor binding to DNA?

A)S1 mapping
B)DNAase I footprinting
C)in vitro mutagenesis
D)S1 mapping and in vitro mutagenesis
E)none of the choices are correct.

Answer

Question 2

(Multiple Choice)

Free
Looking at the binding of proteins to DNA in terms of entropy,it appears that

A)the level of entropy decreases in monomeric proteins promoting binding to DNA.
B)the DNA-protein complex is more ordered than the DNA or protein alone.
C)binding of two protein subunits,independently of each other,causes an increase in entropy.
D)the level of entropy decreases in monomeric proteins promoting binding to DNA and the DNA-protein complex is more ordered than the DNA or protein alone are correct.
E)the DNA-protein complex is more ordered than the DNA or protein alone and binding of two protein subunits,independently of each other,causes an increase in entropy are correct.

Answer

Question 3

(Multiple Choice)

Free
Select the correct statement about enhancers

A)They are proteins that promote transcription of RNA.
B)They stimulate the binding of repressor to DNA.
C)They bind protein factors and stimulate transcription.
D)They are nonpromoter protein elements.
E)None of the choices is correct.

Answer