One approach to prevent religation of the ends of a restricted plasmid DNA is to

A)store the plasmid in DNase.
B)store the plasmid in RNAase.
C)treat the plasmid with alkaline phosphatase to remove phosphates from the 'sticky ends'
D)treat the plasmid with a protease to remove all proteins.
E)treat the plasmid with BamHI to create gaps.