A)135
B)270
C)13,500
D)270,000

A)contains a selectable marker
B)contains an origin of replication
C)has several unique recognition sites for restriction enzymes
D)integrates into the host chromosome
E)can hold an insert of up to 300 kb

A)Restriction endonucleases cleave DNA after particular bases.
B)Fluorescent molecules produce high-energy light that inactivates DNA polymerase.
C)Incorporation of a deoxynucleotide that lacks a base causes DNA polymerase to stall.
D)Incorporation of a dideoxynucleotide that lacks a 3′ hydroxyl.
E)Incorporation of a ribonucleotide that lacks a 5' phosphate.