Analyzing Genomic Variation - Genetics From Genes to Genomes

Allele-specific oligonucleotides (ASO)for the normal and disease alleles of a gene are in one section of a microarray.The disease is a recessive trait.If a probe made from an individual's genomic DNA hybridizes with both ASOs, what can be inferred about the individual?

A)The individual has two normal alleles.
B)The individual has the disease.
C)50% of the individual's children are expected to be carriers.
D)One of the individual's parents has the disease.

Answer

Question 2

(Multiple Choice)

Free

Simple sequence repeats are

A)found only in coding regions of genes.
B)sequences of 1 to 10 base pairs repeated a variable number of times in tandem.
C)sequences that are 10 base pairs long or longer and found in several locations in the genome.
D)members of a gene family.

Answer

Question 3

(Multiple Choice)

Free

The most common mutant allele of the PAH gene, which is responsible for the metabolic disorder PKU, has a SNP in the splice donor site of one intron.What is the simplest way to detect this allele?

A)PCR using genomic DNA as template and two primers, one on either side of the SNP, followed by gel electrophoresis
B)PCR using genomic DNA as template and two primers, one complementary to the region containing the SNP, followed by gel electrophoresis
C)PCR using two primers, one on either side of the SNP, followed by sequencing the PCR products
D)Direct exome sequencing using a microarray
E)Whole-genome sequencing

Answer