In Sanger sequencing, what causes DNA synthesis to terminate at a specific base?

A)Restriction endonucleases cleave DNA after particular bases.
B)Fluorescent molecules produce high-energy light that inactivates DNA polymerase.
C)Incorporation of a deoxynucleotide that lacks a base causes DNA polymerase to stall.
D)Incorporation of a dideoxynucleotide that lacks a 3′ hydroxyl.
E)Incorporation of a ribonucleotide that lacks a 5' phosphate.