Protein Purification And Characterization Techniques - Biochemistry | Biology+

Course

Biochemistry

Study Pack

Set 1: Biochemistry and the Organization of Cells

Set 2: Water: the Solvent for Biochemical Reactions

Set 3: Amino Acids and Peptides

Set 4: The Three-Dimensional Structure of Proteins

Set 5: Protein Purification and Characterization Techniques

Set 6: The Behavior of Proteins: Enzymes

Set 7: The Behavior of Proteins: Enzymes, Mechanisms, and Control

Set 8: Lipids and Proteins Are Associated in Biological Membranes

Set 9: Nucleic Acids: How Structure Conveys Information

Set 10: Biosynthesis of Nucleic Acids: Replication

Set 11: Transcription of the Genetic Code: Biosynthesis of RNA

Set 12: Protein Synthesis: Translation of the Genetic Message

Set 13: Nucleic Acid Biotechnology Techniques

Set 14: Viruses, Cancer, and Immunology

Set 15: The Importance of Energy Changes and Electron Transfer in Metabolism

Set 16: Carbohydrates

Set 17: Glycolysis

Set 18: Storage Mechanisms and Control in Carbohydrate Metabolism

Set 19: The Citric Acid Cycle

Set 20: Electron Transport and Oxidative Phosphorylation

Set 21: Lipid Metabolism

Set 22: Photosynthesis

Set 23: The Metabolism of Nitrogen

Set 24: Integration of Metabolism: Cellular Signaling

Set 5 Protein Purification And Characterization Techniques

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Set 5 Protein Purification And Characterization Techniques

(Multiple Choice)

Free

Which separates on the basis of molecular weight?

A) Gel filtration
B) Affinity chromatography
C) Cation exchange
D) Anion exchange
E) Cation or anion exchange

Answer

(Multiple Choice)

Free

The typical order for the major steps of enzyme isolation would be (from first to last):

A) Homogenization, salt fractionation, electrophoresis, column chromatography.
B) Homogenization, column chromatography, salt fractionation, electrophoresis.
C) Homogenization, salt fractionation, column chromatography, electrophoresis.
D) Salt fractionation, homogenization, electrophoresis, column chromatography.
E) Homogenization, electrophoresis, salt fractionation, column chromatography.

Answer

(Multiple Choice)

Free

Using differential centrifugation it is possible to separate

A) nuclei, mitochondria, and ribosomes into three separate fractions
B) organelles from contaminating salts
C) proteins that differ in charge
D) proteins from membranes

Answer