Course

Biochemistry

Study Pack

Set 13 Nucleic Acid Biotechnology Techniques

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Question 1

(Multiple Choice)

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The application of gel electrophoresis (PAGE) in DNA sequencing

A) is nonexistent because DNA fragments differ widely in charge.
B) depends on separation of similar charge/mass ratio on the basis of size.
C) depends on separation of identical size on the basis of charge/mass ratio.
D) is nonexistent because DNA fragments have no net charge.

Answer

Question 2

(Multiple Choice)

Free
Which of the following statements concerning restriction endonucleases is true?

A) They attack RNA, not DNA.
B) They can produce "sticky ends".
C) They attack single-stranded sequences only.
D) They do not display sequence specificity in their site of attack.

Answer

Question 3

(Multiple Choice)

Free
Restriction enzymes are especially useful for genetic recombination work for all of the following reasons, except:

A) They cut DNA in the middle of specific sequences.
B) They cut DNA independent of the source of the DNA.
C) They often generate single stranded tails or "sticky ends".
D) There are a large variety of them commercially available.
E) All of these traits make restriction enzymes useful.

Answer