The typical order for the major steps of enzyme isolation would be (from first to last):

A) Homogenization, salt fractionation, electrophoresis, column chromatography.
B) Homogenization, column chromatography, salt fractionation, electrophoresis.
C) Homogenization, salt fractionation, column chromatography, electrophoresis.
D) Salt fractionation, homogenization, electrophoresis, column chromatography.
E) Homogenization, electrophoresis, salt fractionation, column chromatography.