During the mating process, yeast cells respond to pheromones secreted by other yeast cells.These pheromones bind GPCRs on the surface of the responding cell and lead to the activation of G proteins inside the cell.When a wild-type yeast cell senses the pheromone, its physiology changes in preparation for mating: the cell stops growing until it finds a mating partner.If yeast cells do not undergo the appropriate response after sensing a pheromone, they are considered sterile.Yeast cells that are defective in one or more components of the G protein have characteristic phenotypes in the absence and presence of the pheromone, which are listed in Table 16-14.
mutation none (wild type) α subunit deleted β subunit deleted γ subunit deleted α and β deleted α and γ deleted β and γ deleted phenotype minus pheromone normal growth arrested growth normal growth normal growth normal growth normal growth normal growth plus pheromone arrested growth, mating response arrested growth, sterile normal growth, sterile normal growth, sterile normal growth, sterile normal growth, sterile normal growth, sterile Table 16-14
Which of the following models is consistent with the data from the analysis of these mutants? Explain your answer.