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Cell Biology

Study Pack

Set 8 Control Of Gene Expression

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Question 1

(Multiple Choice)

Free
Which of the following is not a good example of a housekeeping protein?

A)DNA repair enzymes
B)histones
C)ATP synthase
D)hemoglobin

Answer

Question 2

(Multiple Choice)

Free
Which of the following statements about the Lac operon is FALSE?

A)The Lac repressor binds when lactose is present in the cell.
B)Even when the CAP activator is bound to DNA, if lactose is not present, the Lac operon will not be transcribed.
C)The CAP activator can only bind DNA when it is bound to cAMP.
D)The Lac operon only produces RNA when lactose is present and glucose is absent.

Answer

Question 3

(Multiple Choice)

Free
You are interested in examining the Psf gene.It is known that Psf is normally produced when cells are exposed to high levels of both calcium (Ca2+) and magnesium (Mg2+).MetA, MetB, and MetC are important for binding to the promoter of the Psf gene and are involved in regulating its transcription.MetA binds to the "A" site in the promoter region, MetB to the "B" site, and MetC to the "C" site.You create binding-site mutations in the A, B, and C sites and observe what happens to transcription of the Psf gene.Your results are summarized in Table 8-12. \quad \quad \quad \quad \quad \quad \quad \quad \quad \quad \quad  Transcription in the presence of Ca2+ and/or Mg2+\underline{\text { Transcription in the presence of } \mathrm{Ca}^{2+} \text { and/or } \mathrm{Mg}^{2+}}
 Binding sites present  No Ca2+ or Mg2+ With Ca2+ With Mg2+ With Ca2+ and Mg2 A, B, and C ++ none  A only ++ B only  C only ++ A and B only + A and C only ++++ B and C only +\begin{array}{lcccc}\text { Binding sites present } & \begin{array}{l}\text { No } \mathrm{Ca}^{2+} \\\text { or } \mathrm{Mg}^{2+}\end{array} & \begin{array}{l}\text { With } \\\mathrm{Ca}^{2+}\end{array} & \begin{array}{l}\text { With } \\\mathrm{Mg}^{2+}\end{array} & \begin{array}{l}\text { With } \mathrm{Ca}^{2+} \\\text { and } \mathrm{Mg}^{2 *}\end{array} \\\hline \text { A, B, and C } & - & - & - & ++ \\\hline \text { none } & - & - & - & - \\\hline \text { A only } & - & - & + & + \\\hline \text { B only } & - & - & - & - \\\hline \text { C only } & - & + & - & + \\\hline \text { A and B only } & - & - & - & + \\\hline \text { A and C only } & - & + & + & ++ \\\hline \text { B and C only } & - & - & - & +\end{array}

For this table: -= no transcription of Pst,\text {For this table: -= no transcription of Pst,}
+= low level of transcription of Psf, and\text {\( += \) low level of transcription of Psf, and}
++= high levels of transcription of Pst.\text {\( ++= \) high levels of transcription of Pst.}
Table 8-12
Which of the following proteins are likely to act as gene repressors?

A)MetA only
B)MetB only
C)MetC only
D)both MetA and MetC

Answer