Course
Microbiology
Study Pack
Set 1 The Microbial World
Question 1
(Multiple Choice)
Free
Figure B1.1 represents an artist's attempt to illustrate the process of PCR.What is the most significant error you notice in this figure?
A)DNA strands are not fully denatured
B)Primers are not annealed
C)PCR products cannot separate from template DNA
D)Taq polymerase is not shown in the illustration
E)dNTPs are not shown in the illustration
Answer
Question 2
(Multiple Choice)
Free
If E.coli DNA polymerase,not Taq polymerase were used in polymerase chain reaction assays to amplify SSUrRNA gene sequences,we would expect to see:
A)increased short fragment DNA sequences
B)decreased short fragment DNA sequences
C)the same number of short fragment DNA sequences
D)increasd long fragment DNA sequences
E)decreased long fragment DNA sequences
Answer
Question 3
(Multiple Choice)
Free
A restriction endonuclease cuts DNA at the sequence ACGT.If we assume that all four bases are equally represented in DNA,what are the odds of this sequence occurring on a DNA strand?
A)Roughly every 100 base pairs
B)Roughly every 250 base pairs
C)Roughly every 1000 base pairs
D)Roughly every 10,000 base pairs
E)Roughly every 20,000 base pairs
Answer