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Genetics

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Set 12 Chromosome Chapter Title Tbd

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Question 1

(Multiple Choice)

Free
You want to design a repressor protein mutant. Which protein domain is the best target for preventing binding of the corepressor?

A) activator binding site
B) allosteric domain
C) DNA- binding domain
D) helix- turn- helix domain
E) promoter domain

Answer

Question 2

(Multiple Choice)

Free
Disrupting the formation of which stem- loop structure of trpL would decrease the efficiency of transcriptional regulation?

A) 2- 4 (termination) stem loop
B) 2- 3 (antitermination) stem loop
C) 1- 3 (antitermination) stem loop
D) 1- 2 (pause) stem loop
E) 3- 4 (termination) stem loop

Answer

Question 3

(Multiple Choice)

Free
The electrophoresis gel shown below is from a DNase I footprint analysis of an operon transcription control region. Separate samples of control- region DNA were exposed to DNase I and run in the following lanes: uprotected DNA is in lane 1, DNA protected by repressor is in lane 2, and DNA protected by RNA polymerase is in lane 3. The electrophoresis gel shown below is from a DNase I footprint analysis of an operon transcription control region. Separate samples of control- region DNA were exposed to DNase I and run in the following lanes: uprotected DNA is in lane 1, DNA protected by repressor is in lane 2, and DNA protected by RNA polymerase is in lane 3.   Based on the gel, what nucleotide positions are occupied by the repressor and RNA polymerase? A) 19 through 29 by the repressor and 7 through 24 RNA polymerase B) 1 through 18 by the repressor and 1 through 6 by RNA polymerase C) 30 through 35 by the repressor and 7 through 24 by RNA polymerase D) 1 through 18 by the repressor and 25 through 35 by RNA polymerase E) 19 through 29 by the repressor and 1 through 6 by RNA polymerase Based on the gel, what nucleotide positions are occupied by the repressor and RNA polymerase?

A) 19 through 29 by the repressor and 7 through 24 RNA polymerase
B) 1 through 18 by the repressor and 1 through 6 by RNA polymerase
C) 30 through 35 by the repressor and 7 through 24 by RNA polymerase
D) 1 through 18 by the repressor and 25 through 35 by RNA polymerase
E) 19 through 29 by the repressor and 1 through 6 by RNA polymerase

Answer