A) The gene of interest is typically inserted in the vector plasmid between the P element inverted repeat end sequences.
B) The selectable marker is typically inserted in the vector plasmid between the P element inverted repeat end sequences.
C) The transposase activity inserts P elements into the genome at specific positions in the genome.
D) P elements transpose only in the germ- line cells of Drosophila.
E) A second plasmid can be used to supply the transposase activity in trans.

A) altered codon usage within the heterologous sequences to approximate the codon bias in E. coli
B) the use of cDNAs, that are free of introns, as eukaryotic transgenes
C) an E. coli expression vector with a promoter sequence that binds RNA polymerase
D) an E. coli expression vector with a Shine- Dalgarno sequence for efficient translation
E) a negative selectable marker to select against nonhomologous recombination events

A) Some donor pieces will remain uninserted.
B) Contamination will have introduced other donor inserts.
C) Some donor inserts will be sensitive to particular antibiotics.
D) Some donor strands will be inserted with an incorrect orientation.
E) Some donor inserts will be single stranded and deteriorate.