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Genetics

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Set 6 Genetic Analysis And Mapping In Bacteria And Bacteriophage

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Question 1

(Multiple Choice)

Free
To map genes of a bacterial strain, conjugation must be interrupted at given times. Suppose you have Hfr cells of genotype a+b+c+d+e+strR and F- cells of genotype a- b- c- d- e- strS and you combine these two cultures in liquid medium in four blenders at time 0. After intervals of 3, 6, 9, and 12 minutes, you turn on successive blenders. The graph below shows the results of plating the cultures from each blender. What is the correct order of the genes on this chromosome? To map genes of a bacterial strain, conjugation must be interrupted at given times. Suppose you have Hfr cells of genotype a+b+c+d+e+strR and F- cells of genotype a- b- c- d- e- strS and you combine these two cultures in liquid medium in four blenders at time 0. After intervals of 3, 6, 9, and 12 minutes, you turn on successive blenders. The graph below shows the results of plating the cultures from each blender. What is the correct order of the genes on this chromosome?   A) a b c d e B) b e c a d C) a c e b d D) d a c e b E) b e c a (d is unknown)

A) a b c d e
B) b e c a d
C) a c e b d
D) d a c e b
E) b e c a (d is unknown)

Answer

Question 2

(Multiple Choice)

Free
A lab technician has four strains of bacteria. Although he forgot to label them, he knows that they include one F+ strain, one Hfr strain, one F' strain, and one F- strain. He observed the following results after plating these strains on different media. Which of the following plates can be used to identify the F- strain?  strain  his g gu  cys  leu  str F+++++Hfr++++F++++F++\begin{array} { | l l l l l l | } \hline \text { strain } & \text { his } & g \text { gu } & \text { cys } & \text { leu } & \text { str } \\\mathrm { F } ^ { + } & + & + & + & - & + \\\mathrm { Hfr } & + & + & + & + & - \\\mathrm { F } ^ { \prime } & + & + & + & + & - \\\mathrm { F } & - & - & - & + & + \\\hline\end{array}

A) minimal media with leucine and streptomycin
B) minimal media with histidine, glutamate, and cysteine
C) minimal media with histidine, glutamate, cysteine, and streptomycin
D) complete media
E) minimal media with streptomycin

Answer

Question 3

(Multiple Choice)

Free
In five Hfr strains, each of which was used to build a time- of- entry map, the genes entered the recipient cells as follows:
Strain 1: S L A C T F
Strain 2: N P F T C A
Strain 3: T F P N U Y
Strain 4: S H Y U N P
Strain 5: U N P F T C

Which of the following represents a correct compilation of these results

A) N P F T S L A C H U T
B) T C A L S P N U Y H
C) S L A C T F P N U Y H
D) S L A C T F P N H C U
E) U N P F T C A L S T F

Answer