Course

Genetics

Study Pack

Set 17 Recombinant Dna Technology

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Question 1

(Multiple Choice)

Free
A ddNTP, used often in DNA sequencing, lacks a(n) carbons.

A) OH; 2'; 3'
B) carboxyl; 5'; 3'
C) methyl; 2'; 3'
D) OH; 2'; 5'
E) None of the listed answers is correct.

Answer

Question 2

(Essay)

Free
Molecular biologists rely on many, often sophisticated, techniques to pursue their
discipline. Ultracentrifugation, electron microscopy, X -ray diffraction, electrophoresis, and computer interfacing are fundamental tools. Model organisms provide the raw materials for study. List three organisms (or organismic groups) often used by
recombinant DNA technologists and describe a major advantage of each group.

Answer

Bacteriophage: useful vector, relatively...

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Question 3

(Multiple Choice)

Free
Assume that a plasmid (circular) is 3200 base pairs in length and has restriction sites at the following locations: 400, 700, 1400, 2600. Give the expected sizes of the restriction fragments following complete digestion.

A) 300, 700, 2200
B) 400, 800, 1000 (2 of these)
C) 400, 1200, 1600
D) 300, 700, 1000, 1200
E) 700, 400, 1400, 2600

Answer