Genomics And Proteomics - Genetics A Conceptual Approach

Crossing over is often reduced around centromeric regions of chromosomes. If you were trying to construct a genetic map of two linked marker loci in this region, what result might you obtain?

A) The recombination frequencies would be low, and you would deduce that the markers were very close to one another.
B) The recombination frequencies would be low, and you would deduce that the markers were very far from one another.
C) The recombination frequencies would be high, and you would deduce that the markers were very close to one another.
D) The recombination frequencies would be high, and you would deduce that the markers were very far from one another.
E) The recombination frequencies would be too low to detect, and you would not be able to estimate the distance between genetic markers.

Answer

Question 2

(Multiple Choice)

Free

For a genetic map of a chromosome, distances are measured in units of:

A) restriction sites.
B) RFLPs.
C) centiMorgans.
D) base pairs.
E) contigs.

Answer

Question 3

(Multiple Choice)

Free

You've cloned a 2-kb fragment of DNA into a bacterial cloning vector and want to construct a restriction map of the insert. You amplify the 2-kb insert using PCR, purify it, and subject it to differential digestion with the enzymes EcoRI and HindIII, gel-fractionate the digests, and visualize the restriction patterns by staining the gels with ethidium bromide to generate the following results.
-Using these data, indicate the order of restriction sites in the DNA insert.

\begin{array} { l l } & \text { Restriction fragment lengths } \\\hline\text { EcoRI } & 350 \mathrm { bp } , 500 \mathrm { bp } , 1250 \mathrm { bp } , 100 \mathrm { bp } \\\text { HindIII } & 350 \mathrm { bp } , 650 \mathrm { bp } , 1000 \mathrm { bp } \\\text { EcoRI/HindIII } & 100 \mathrm { bp } , 150 \mathrm { bp } , 200 \mathrm { bp } , 300 \mathrm { bp } , 350 \mathrm { bp } , 900 \mathrm { bp }\end{array}

A) EcoRI, HindIII, EcoRI, HindIII, EcoRI
B) HindIII, EcoRI, HindIII, EcoRI, HindIII
C) HindIII, EcoRI, HindIII, HindIII, EcoRI
D) HindIII, EcoRI, EcoRI, HindIII, EcoRI
E) EcoRI, EcoRI, HindIII, EcoRI, HindIII

Answer