All of the following are requirements of a bacterial cloning vector EXCEPT:
A)origin of replication. B)unique restriction enzyme sites. C)Ti plasmid. D)selectable markers. E)All of these are requirements of a bacterial cloning vector.
The restriction enzyme Pst1 cleaves phosphodiester bonds between the G and the A nucleotides in its recognition sequence which is 5' CTGCAG 3' for one of the two DNA strands.After cutting by Pst1, the resulting DNA fragments will have:
A)3' single-stranded overhanging ends (end in a 3' group). B)5' single-stranded overhanging ends. C)blunt ends. D)3' P groups. E)both 5'single-stranded overhanging ends and 3' P groups.