Question 2

(Multiple Choice)

The restriction enzyme Pst1 cleaves phosphodiester bonds between the G and the A nucleotides in its recognition sequence which is 5' CTGCAG 3' for one of the two DNA strands.After cutting by Pst1, the resulting DNA fragments will have:


A)3' single-stranded overhanging ends (end in a 3' group).
B)5' single-stranded overhanging ends.
C)blunt ends.
D)3' P groups.
E)both 5'single-stranded overhanging ends and 3' P groups.

Answer

A