The discovery of green fluorescent protein (GFP)has greatly facilitated living cell experiments because:
A)GFP is green. B)GFP requires a jellyfish-specific cofactor. C)GFP sequences may be readily fused to those of other proteins. D)wild-type GFP folding is adapted to normal seawater temperatures,15-25 °C.
Given the wild type (normal)yeast on the left and the mutant yeast on the right,identify the defective phenotype for the mutant.
A)entry into the ER B)fusion of transport vesicles from the ER to the Golgi C)budding from the Golgi to secretory vesicles D)fusion of secretory vesicles with the plasma membrane