Course

Microbiology

Study Pack

Set 12 Biotechniques And Synthetic Biology

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Question 1

(Multiple Choice)

Free
The genes for proteins such as b-galactosidase (lacZ) or green fluorescent protein (gfp) can be fused to the promoter of a gene of interest. Which of the following statements is NOT correct about gfp and lacZ?

A) They are called reporter genes.
B) The enzymatic activity of b-galactosidase can be easily assayed.
C) GFP is visualized by fluorescence microscopy.
D) Both genes are of viral origin.
E) The gfp gene is of eukaryotic origin, whereas lacZ is a bacterial gene.

Answer

Question 2

(Multiple Choice)

Free
A common approach to study a certain metabolic process is to isolate organisms defective in that particular process. These organisms are called

A) agents.
B) wild type.
C) prototrophs.
D) mutants.
E) vectors.

Answer

Question 3

(Multiple Choice)

Free
Which of the following is correct regarding operon fusions?

A) They expose both transcriptional and translational controls.
B) Both target and reporter genes have their own promoter sequences.
C) Both target and reporter genes have ribosome-binding sites.
D) Only the reporter gene has a promoter.
E) They produce a single transcript and a single hybrid reporter-target polypeptide.

Answer