Which Compound is NOT Required for Initiation of DNA Replication

Question

Which compound is NOT required for initiation of DNA replication in E. coli?
A) DnaB (helicase)
B) DnaG (primase)
C) Dam methylase
D) DNA ligase
E) DnaA (a AAA+ ATPase)

Accepted Answer

DNA ligase is not required for initiation of DNA replication in E. coli. It is involved in the later stages of replication, specifically in joining Okazaki fragments on the lagging strand.