The Sanger Method of Sequencing Uses ALL of the Following

Question

The Sanger method of sequencing uses ALL of the following EXCEPT:
A)dideoxynucleotides.
B)DNA polymerase.
C)a short oligo primer.
D)deoxynucleotides
E)restriction enzymes.

Accepted Answer

The Sanger method of sequencing uses dideoxynucleotides, DNA polymerase, and a short oligo primer to create fragments of DNA that differ in length by one nucleotide. The method does not involve the use of restriction enzymes.