Ion Torrent Sequencing Detects

Question

Ion torrent sequencing detects:
A)electrons liberated as nucleotides are incorporated into DNA
B)protons liberated as nucleotides are incorporated into DNA
C)pyrophosphate liberated as nucleotides are incorporated into DNA
D)the nucleotide found at the 3' end of a DNA fragment
E)the nucleotide found at the 5' end of a DNA fragment

Accepted Answer

Ion torrent sequencing detects protons (H+) that are released as a byproduct when nucleotides are incorporated into a growing DNA strand. This change in pH is measured and used to determine the sequence of the DNA.