In Pyrosequencing,which Enzyme Liberates Pyrophosphate

Question

In pyrosequencing,which enzyme liberates pyrophosphate?
A)DNA polymerase
B)Luciferase
C)ATP sulfurylase
D)Restriction endonuclease
E)DNA ligase

Accepted Answer

DNA polymerase is responsible for adding nucleotides to a growing DNA strand during pyrosequencing, and the addition of each nucleotide releases pyrophosphate (PPi) as a by-product.