Which of the following is not an assumption when calculating volumes of distribution using the Fick dilution principle?
A) The concentration of the solute is homogeneous among all its volume of distribution. B) The amount of solute is known at the time of sampling of the volume in which it is distributed. C) The volume in which the solute is distributed is well mixed. D) The solute’s concentration can be determined. E) The solute is small.
You have a 0.1 M solution of ATP and take 6.25 mL of it to add to a 25 mL volumetric flask, along with some other solutions, which is then brought up to volume to make a stock solution. You then add 1 mL of that stock solution to a reaction bath that has a total final volume, including the 1 mL of stock solution, of 5 mL. W hat is the ATP concentration in the final reaction bath?
A) 0.025 M B) 0.05 M C) 0.02 M D) 0.005 M E) Cannot be calculated from the information given.