Course

Cell Biology

Study Pack

Set 6 Polymerase Chain Reaction

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Question 1

(Multiple Choice)

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In real time PCR,the presence of double-stranded DNA is detected byــــــــــــــــــــــــ .

A)fluorescence of SYBR Green
B)luminescence of SYBER Green
C)quenching of SYBR Green fluorescence
D)rapid decrease in the amount of SYBR Green fluorescence

Answer

Question 2

(Multiple Choice)

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The enzyme that converts RNA back into a DNA copy called complementary DNA cDNA)is calledـــــــــــــــــــــــ .

A)DNA polymerase
B)RNA polymerase
C)cDNA polymerase
D)Reverse transcriptase

Answer

Question 3

(True False)

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RNA can be synthesized in PCR if RNA polymerase and RNA nucleotides are used in place of DNA polymerase and DNA nucleotides,respectively.

Answer