Course

Microbiology

Study Pack

Set 11 Applications Of Molecular Diagnostics

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Question 1

(Multiple Choice)

Free
The principle of in-solution hybridization is

A) the hybridization between a labeled probe and target nucleic acids in a liquid solution in tubes or in microtiter wells.
B) the hybridization between an immobilized labeled probe and target nucleic acids in a liquid solution in tubes or in microtiter wells.
C) the hybridization between a labeled probe in solution and immobilized target nucleic acids.
D) when two solutions are combined and hybridization occurs between the probe and the target, then the tube is centrifuged to detect the precipitated hybridized target and probes.

Answer

Question 2

(Multiple Choice)

Free
How do higher probe concentrations affect a given hybridization reaction?

A) Increase the temperature
B) Lower the reaction time
C) Lower the ionic strength
D) Increase the pH

Answer

Question 3

(Multiple Choice)

Free
Common uses of probes include all of the following, except

A) microbial pathogen detection.
B) gene expression analysis.
C) abnormal white blood cell (WBC) detection.
D) chromosomal translocations.

Answer