Course

Microbiology

Study Pack

Set 17 Recombinant Dna Technology

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Question 1

(Multiple Choice)

Free
Which cloning vector should be used to express a 500-kb DNA fragment in Saccharomyces cerevisiae?

A)YAC
B)BAC
C)pUC19
D) λ\lambda 1059

Answer

Question 2

(Multiple Choice)

Free
Which is most analogous to the role of GFP in recombinant DNA technology?

A)Using dyes to stain and detect bacteria under light microscopy from the sputum of a patient diagnosed with tuberculosis.
B)Comparing the RFLP patterns of DNA from various suspects to DNA isolated from the scene of a crime using gel electrophoresis.
C)Creating a metagenomic library representing archaea bacteria that thrive in the hot springs of Yellowstone National Park.
D)Monitoring the spread of multi-drug resistant pathogens on a hospital floor by comparing antibiotic susceptibility patterns.

Answer

Question 3

(Multiple Choice)

Free
Which of the following components of PCR ensures amplification of the target DNA sequence only,despite the presence of many other DNA sequences?

A)Primers
B)Polymerases
C)dNTPs
D)High temperatures

Answer