A) Heat to 70°C (to bind primers and enzymes), then cool to 50°C (to synthesize DNA), and then reheat to 90°C (to separate DNA).
B) Heat to 70°C (to separate DNA), then cool to 50°C (to bind primers and enzymes), and then reheat to 90°C (to synthesize DNA).
C) Heat to 90°C (to separate DNA), then cool to 50°C (to bind primers and enzymes), and then reheat to 70°C (to synthesize DNA).
D) Heat to 90°C (to bind primers and enzymes), then cool to 50°C (to separate DNA), and then reheat to 70°C (to synthesize DNA).