Select possible outcomes after RISC binds to an mRNA during RNA interference. Choose all that apply.
A) Inhibition of translation without mRNA degradation B) mRNA degradation C) Movement of the RISC-mRNA complex to a P-body D) Expulsion of the RISC-mRNA complex from the cell E) Cell death
You are working in a lab that studies ncRNAs. You develop an assay to isolate ncRNAs and any associated molecules. You must then identify the molecules you have isolated. You find a molecule that is associated with an ncRNA that is not changed by protease, RNase, or DNase. The associated molecule is most likely
A) a small molecule. B) a protein. C) an mRNA. D) DNA.
You are working in a lab that studies snoRNAs. You mutate the antisense sequences in a C/D box snoRNA to completely different sequences. What results do you expect compared to wild type snoRNA? Check all that apply.
A) Reduced binding of the snoRNA to the rRNA B) Reduced methylated ribose in the rRNA C) Reduced psuedouracil in the rRNA D) Increased binding of the snoRNA to the rRNA E) Increased methylated ribose in the rRNA