Course

Genetics

Study Pack

Set 10 Recombinant Dna Technology

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Question 1

(Multiple Choice)

Free
Which of the following techniques is best suited to study protein interactions?

A)PCR
B)The yeast two-hybrid system
C)Restriction mapping
D)RT-PCR
E)Southern blotting

Answer

Question 2

(Multiple Choice)

Free
RFLPs arise due to

A)changes in the number of tandem repeats.
B)base changes that add or delete restriction enzyme recognition sites.
C)addition or deletion of bases between restriction enzyme recognition sites.
D)B and C only
E)All of these

Answer

Question 3

(Multiple Choice)

Free
Successful cotransformation in the yeast two-hybrid system will result in the binding of proteins produced by each yeast expression vector.These fusion proteins function by

A)binding to the lacZ operator,preventing transcription.
B)binding to RNA polymerase,preventing transcription.
C)binding to the lacZ repressor,enabling transcription.
D)binding to the lacZ upstream activator sequence,enabling transcription.
E)changing color in the presence of X-gal.

Answer