Which Reaction in Glycolysis Utilizes a Covalent Enzyme Intermediate

Question

Which reaction in glycolysis utilizes a covalent enzyme intermediate?
A) phosphofructokinase-1
B) glyceraldehyde-3-phosphate dehydrogenase
C) phosphohexose isomerase
D) enolase
E) triose phosphate isomerase

Accepted Answer

Glyceraldehyde-3-phosphate dehydrogenase utilizes a covalent enzyme intermediate in which an oxidation-reduction reaction takes place, leading to the formation of NADH and a high-energy phosphorylated intermediate. The other reactions in glycolysis do not involve a covalent enzyme intermediate.