E. Coli DNA Polymerase III

Question

E. coli DNA polymerase III:
A) can initiate replication without a primer.
B) is efficient at nick translation.
C) is the principal DNA polymerase in chromosomal DNA replication.
D) represents over 90% of the DNA polymerase activity in E. coli cells.
E) requires a free 5'-hydroxyl group as a primer.

Accepted Answer

DNA polymerase III is the main enzyme responsible for DNA replication in E. coli, synthesizing the new DNA strands by adding nucleotides to a pre-existing primer.