Which DNA Primer Would Have the HIGHEST Melting Temperature

Question

Which DNA primer would have the HIGHEST melting temperature?
A) GCATCGGC
B) AATCGGAT
C) ATACAGATCGGC
D) ACCGGCAGGTCGGC
E) ATACGCAGATCGGC

Accepted Answer

The melting temperature of a DNA primer is influenced by its length and GC content. Primer D has the highest GC content and is one of the longer options, both of which contribute to a higher melting temperature.