Which of the Following Would Be MOST Appropriate for Cloning

Question

Which of the following would be MOST appropriate for cloning a gene that is 225 kb in size?
A)Plasmid
B)Cosmid
C)Phage lambda
D)BAC
E)Yeast phage

Accepted Answer

BAC (Bacterial Artificial Chromosome) is the best choice for cloning a gene that is 225 kb in size as it can accommodate inserts up to 300 kb in size and is more stable than other vectors such as plasmids, cosmid, or phage lambda. Yeast phage is not commonly used for cloning large genes.