Responsible for the Majority of DNA Replication

Question

Responsible for the majority of DNA replication.
A) DNA ligase
B) DNA primase
C) Topoisomerase
D) DNA polymerase I
E) DNA polymerase III

Accepted Answer

DNA polymerase III is the main polymerase enzyme responsible for adding nucleotides to the new strand during DNA replication. It is highly processive, meaning it can catalyze the addition of many nucleotides in a row without dissociating from the template strand. While other enzymes such as DNA polymerase I, DNA primase, and DNA ligase are also involved in DNA replication, they play supporting roles rather than being the primary polymerase enzyme. Topoisomerase helps to untangle the DNA ahead of the replication fork, but does not directly participate in DNA synthesis.