Which of the Following Enzyme Activities is NOT Used in Base

Question

Which of the following enzyme activities is NOT used in base excision repair?
A) Glycosylase
B) Apyriminidic endonuclease
C) Deoxyribose-5'-phosphatase
D) DNA polymerase
E) Helicase

Accepted Answer

Helicase is not used in base excision repair. The other enzymes mentioned (glycosylase, apyriminidic endonuclease, deoxyribose-5'-phosphatase, and DNA polymerase) are all involved in various steps of base excision repair. Glycosylase recognizes and removes damaged bases, apyriminidic endonuclease cleaves the DNA backbone, deoxyribose-5'-phosphatase removes the sugar moiety, and DNA polymerase fills in the gap with the correct nucleotides. Helicase is not needed in this process as it unwinds DNA during replication and transcription.