Citrate Synthase Has a ____ ΔG°′ and is Essentially ____

Question

Citrate synthase has a ____ ΔG°′ and is essentially ____ due to the ____ of the CoA thioester.
A) large positive; reversible; oxidation
B) nearly zero; reversible; reduction
C) nearly zero; irreversible; reduction
D) large negative; irreversible; hydrolysis
E) large negative; reversible; hydrolysis

Accepted Answer

Citrate synthase has a large negative ΔG°′, indicating that the reaction is thermodynamically favorable. The reaction is irreversible due to the hydrolysis of the CoA thioester bond, which is a high-energy bond.