Which of the Following Uses an RNA Template During the Technique

Question

Which of the following uses an RNA template during the technique?
A)TA cloning
B)RAPD
C)Inverse PCR
D)RT-PCR

Accepted Answer

RT-PCR, or Reverse Transcriptase Polymerase Chain Reaction, uses an RNA template to create a complementary DNA (cDNA) strand through the action of reverse transcriptase, before amplifying the sequence using PCR. The other techniques listed do not utilize an RNA template.