What is the Function of the RNA Primer During DNA

Question

What is the function of the RNA primer during DNA synthesis?
A)DNA polymerase requires a 3' hydroxyl to initiate synthesis.
B)The primer helps the replication machinery find the origin of replication.
C)The primer is required for cleavage of the DNA to generate a loose end.
D)The primer signals DNA polymerase to terminate synthesis.

Accepted Answer

During DNA synthesis, DNA polymerase requires a 3' hydroxyl group to initiate synthesis. However, DNA does not have a 3' hydroxyl group available for the polymerase to attach to, so an RNA primer with a 3' hydroxyl group is synthesized by primase to initiate DNA synthesis. Once the primer is in place, DNA polymerase can attach and elongate the DNA strand.