Which of the Following is NOT Used to Measure the Decay

Question

Which of the following is NOT used to measure the decay of an mRNA transcript?
A)actinomycin D
B)α-amanitin
C)5,6-dichloro-1-β-D-ribobenzimidazole (DRB)
D)puromycin

Accepted Answer

Puromycin is not used to measure the decay of an mRNA transcript. It is a translation inhibitor that works by mimicking aminoacyl-tRNA and prematurely terminating translation. The other three options listed (actinomycin D, α-amanitin, and DRB) are all inhibitors of transcription and are commonly used to study mRNA decay.