How are Hybrid Proteins or Fusion Proteins Produced

Question

How are hybrid proteins or fusion proteins produced?
A)by incubation of 2 proteins with a protease
B)by expression of genes coding for multiple proteins
C)by translation of mRNAs without removing exons
D)by translation of recombinant sequences from expression vectors carrying cDNA inserts cloned directly into the coding sequence of a vector-born protein-coding gene

Accepted Answer

Hybrid or fusion proteins are produced by translation of recombinant sequences from expression vectors carrying cDNA inserts cloned directly into the coding sequence of a vector-born protein-coding gene. This technique results in the production of a single protein containing two or more different functional domains from different proteins.