Which of the Following is NOT Correct for CDNA Libraries

Question

Which of the following is NOT correct for cDNA libraries?
A)Reverse transcriptase synthesizes a DNA strand complement of the mRNA templates.
B)mRNA templates are isolated using oligo (dA)-cellulose chromatography.
C)Linkers are added and the cDNA is cloned into suitable vectors.
D)The cDNA are copies from mRNA templates.

Accepted Answer

cDNA libraries are usually constructed from mRNA molecules obtained from the cells or tissues of interest. The mRNA is isolated using poly(A) tail-specific oligonucleotide probes, not oligo(dA)-cellulose chromatography. The mRNA is then used as a template to produce a complementary DNA (cDNA) copy using the enzyme reverse transcriptase. The resulting cDNA is then cloned into a suitable vector, often after the addition of linkers. Therefore, option B is not correct because it suggests the use of a different method for mRNA isolation than the one commonly used for cDNA library construction.