Question 5

(Multiple Choice)

Which of the following regarding the hydrolysis of p-nitrophenyl acetate by chymotrypsin is correct?


A)The active site contains a serine residue,an unprotonated histidine,and an unprotonated aspartate residue at pH 7.0 prior to binding the substrate.
B)The first product is acetic acid.
C)An actyl-enzyme tetrahedral intermediate forms during the mechanism.
D)Attack of a water molecule on the acyl-enzyme intermediate yields the first product.

Answer