In Affinity Chromatography, a Protein

Question

In affinity chromatography, a protein
A) which binds to the ligand will remain on the column.
B) which binds to the ligand will elute from the column.
C) which is hydrophobic will remain on the column.
D) which is hydrophilic will remain on the column.

Accepted Answer

Affinity chromatography works by using a ligand that is specific for the protein of interest. The ligand is attached to a solid support matrix, and a protein sample is loaded onto the column. Proteins that do not bind to the ligand will flow through the column, while the protein of interest, which binds to the ligand, will remain on the column. The protein can then be eluted from the column by washing with a buffer that disrupts the specific interaction between the ligand and the protein. Therefore, A is the correct choice as it describes the key step of the affinity chromatography process.