Which of the Enzymes Covalently Connects Segments of DNA

Question

Which of the enzymes covalently connects segments of DNA?
A)helicase
B)topoisomerase
C)DNA ligase
D)single-stranded binding protein
E)primase

Accepted Answer

DNA ligase is the enzyme responsible for covalently connecting segments of DNA by catalyzing the formation of phosphodiester bonds between adjacent nucleotides. Helicase unwinds the double-stranded DNA and separates the two strands, while topoisomerase helps to relieve the supercoiling that occurs ahead of the replication fork. Single-stranded binding protein binds to single-stranded DNA and prevents reannealing of the strands, while primase synthesizes short RNA primers that serve as starting points for DNA synthesis by DNA polymerase. None of these enzymes are directly involved in covalently connecting segments of DNA.